FastQCFastQC Report
Mon 27 Feb 2023
SRR3128966.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128966.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1560204
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128620.824379376030314No Hit
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC74650.4784630727776624Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG63060.4041779151957052No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG50010.3205350069606282No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT34980.2242014505795396No Hit
AGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT21980.14087901325724073No Hit
GGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT21740.13934075287590597No Hit
ACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT15750.10094833752509287No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50250.080.4331051
GGTAAGG7350.078.740011
ATAGCGG4150.078.230971
AGGGAAT17200.076.781095
AGTAAGG9350.075.987661
TAGGGAT8900.073.9289254
AGATAGG7700.073.327731
ATAGGGA12900.072.864623
TAAGCGG4200.072.818511
AAGGGTA8700.072.387234
ATAGGGC9200.072.029053
GAATAGG8500.071.961821
AGGGATG25350.071.933335
TATAGGG9700.071.266672
TAAGGGA12750.071.141583
GTAGGGC8200.071.069663
ACTAGGG8050.070.685512
GACTAGG3600.070.577941
TAGGGCA6950.070.327464
CGTAGGG4100.069.965972