FastQCFastQC Report
Mon 27 Feb 2023
SRR3128965.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128965.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641086
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134780.8212854170957524No Hit
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC80630.4913209910997961Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG66870.40747407509417544No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG56090.34178586618860923No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT37030.22564326305873064No Hit
AGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT23360.1423447643816351No Hit
GGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT22260.13564188592188345No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG4050.081.3101651
CGTTTTT55150.079.6715241
GGTAAGG9650.079.462481
CGTAGGG4400.079.037052
AAGGGTA10400.074.559384
AGTAAGG12500.074.140931
AGGGATG23250.073.979235
TAGGGCA6900.073.5573964
AGGGAAT19700.072.758765
ATAGGGT5100.071.874713
AGATAGG8000.070.5656051
ATAGAGG9350.069.433531
TAGAGGG26100.069.322132
GAATAGG9450.069.196621
AAGGGAT22900.069.1585854
TAAGGGA14550.068.796813
AGAGGGC22700.068.525753
GTAGGGC8600.068.306693
GATTAGG4550.068.2392651
ATAAGGG23300.068.173012
Produced by FastQC (version 0.11.5)