FastQCFastQC Report
Mon 27 Feb 2023
SRR3128964.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128964.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2544224
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166060.6526941024060775No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG41960.1649225854327292RNA PCR Primer, Index 38 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC37180.14613493151546406RNA PCR Primer, Index 38 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG35590.13988548178147836RNA PCR Primer, Index 27 (95% over 22bp)
GGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAA29310.1152021205680003No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76150.080.769511
ATAGGGC13650.072.8830953
GGTAAGG15200.070.440441
TAGGGCA12800.070.355894
ATGCGGG10300.070.03762
CGAAGGG24850.069.822992
CGTAGGG8650.069.770352
AGGGTAC13700.069.631815
ACGGGTA3900.068.9108054
TAGGGTA7750.068.7474
GGGCGAT15850.068.488076
GTAGGGC12800.067.77743
AGTAGGG41700.067.3887252
TAGACGG2950.067.153251
AAGGGAT41450.067.112674
TAGAGGG47400.066.546442
ATAGCGG3900.066.51781
AAGGGTA18700.065.303314
ATAGGGA22700.065.012293
ATGAGGG36450.064.9356462
Produced by FastQC (version 0.11.5)