FastQCFastQC Report
Mon 27 Feb 2023
SRR3128963.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128963.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2601332
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161350.6202591595382673No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG43840.1685290458887985RNA PCR Primer, Index 38 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC39970.15365205210253824RNA PCR Primer, Index 38 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG37190.14296521935685255RNA PCR Primer, Index 27 (95% over 22bp)
GGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAA29730.11428760342778238No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79000.080.247811
TAGGGCA12600.073.108584
ATAGGGC14750.071.37383
CGTAGGG7050.069.380282
AGGGTAC12400.069.3605045
GTAGGGC12350.068.11913
AGTAGGG44450.067.717372
GGTAAGG14750.067.326041
CGAGGGA15500.067.3138053
ATAGCGG4550.067.234881
CGAAGGG23950.066.963892
ATAAGGG35300.066.484082
TAAGGGA22350.066.44963
AAGGGTA17400.066.445984
GGGCGAT16800.066.021536
AGGGATG37900.065.971335
AAGGGAT41750.065.628864
TAGGGTA6950.064.918624
AAGGGAC26050.064.7693564
GTAGGGA21200.064.733613
Produced by FastQC (version 0.11.5)