FastQCFastQC Report
Mon 27 Feb 2023
SRR3128962.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128962.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2691555
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166960.6203105639676693No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG45140.16770974399557134RNA PCR Primer, Index 38 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC41970.15593216560687037RNA PCR Primer, Index 38 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG39030.14500911183312248RNA PCR Primer, Index 27 (95% over 22bp)
GGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAA29980.11138542589692575No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT73500.081.2400361
ATAGGGC14500.072.926543
CGTAGGG8450.071.190872
AGGGTAC13000.070.131535
GGTAAGG17900.069.9237061
AAGGGAT44700.069.812254
ATAGCGG5150.069.43881
AGTAGGG45250.068.340542
GTAGGGC10700.068.079923
TAGGGCA12850.067.6611944
AAGGGCG12450.066.8151554
GGGCGAT18250.066.693546
TAAGAGG19350.066.6293261
CGAAGGG24300.066.337472
AAGGGTA19250.066.1621554
ATAAGGG37100.065.998632
TATAGGG18500.065.7959442
AGGGAAT32050.065.397595
TAAGGGA22650.065.360213
TAGGGTA8300.065.116474
Produced by FastQC (version 0.11.5)