FastQCFastQC Report
Mon 27 Feb 2023
SRR3128961.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128961.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1750435
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230751.3182437508390772No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG19190.10962989199827472No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT97750.085.701281
TAGGGCA8800.073.9427644
GTAGGGC7950.071.76583
CGTAGGG5600.071.569862
AGGGATG27250.071.2223745
ATAGAGG11500.070.138971
AGTAGGG27000.069.8546752
ATAGGGC10600.069.83823
TAGAGGG32000.068.812312
ATAGGGA15650.068.694153
GTAGGGA11500.066.012673
TAAGGGA15800.065.952993
AGAGGGC26800.065.8015443
TATAGGG13500.065.3141252
TAGGGCG4350.065.037114
AAGGGAT27750.064.908214
GAGGGTA9450.064.865054
AATGCGG3200.064.858041
AGGGTAC7050.064.6575
AAGGGCA27300.064.596384
Produced by FastQC (version 0.11.5)