FastQCFastQC Report
Mon 27 Feb 2023
SRR3128960.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128960.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1784762
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224121.2557416619134651No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG19920.11161152019148772No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95650.085.387841
AGGGATG27250.071.747665
AGTAGGG28050.071.079622
ATAGGGA15550.070.119453
TAGGGCA7650.070.036484
ATAACGG1700.069.186661
TAGAGGG32650.069.130582
ACGGGAT5000.068.6173254
AGGGTAC8950.068.265495
TAGGGAT11850.068.216744
TAAGGGA15950.067.476993
GGTAAGG10850.067.20991
AAGGGTA11000.066.2246864
TATAGGG14150.065.799242
CGTAGGG5750.065.423572
GTAGGGC7400.065.416373
ATAGGGC10000.065.327453
GAGGGTA10050.064.0671544
TAGACGG1700.063.651731
ATGTAGG6800.063.651731
Produced by FastQC (version 0.11.5)