FastQCFastQC Report
Mon 27 Feb 2023
SRR3128959.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128959.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908580
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229311.201469155078645No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG19380.10154146014314308No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT99200.085.751091
ATAGGGC10550.071.713783
ACGGGTA1000.070.488864
AGTAGGG31200.070.185782
ATAAGGG26050.068.728222
TAGGGCA9850.068.69984
TAAGGGA17150.068.228293
ATAGGGA16050.067.048593
TATAGGG15750.066.832132
GACCGAT1550.066.6991358
TAGAGGG35950.066.402232
CGTAGGG6700.065.928152
AAGAGGG72350.065.924272
GTAGGGA14200.065.855783
AGGGATG27650.065.6026465
AGTAAGG11550.065.583841
GTAGGGC9100.065.066643
AAGGGGC55850.065.040764
AAGGGAC20550.064.9435044
GGAGCTA36950.064.861199
Produced by FastQC (version 0.11.5)