FastQCFastQC Report
Mon 27 Feb 2023
SRR3128958.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128958.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2205754
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT375531.7025017295673046No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG29920.135645226076888No Hit
CGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT24810.11247854475159062No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC22460.10182459150023075No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT152900.087.088361
ATAGGGC11600.072.374383
AGGGATG39350.072.0107965
TAGGGCA10350.070.178364
ATAGGGA20600.069.145293
AGGGTAC10600.068.71495
ATAGCGG3650.068.516261
GTAGGGC10450.067.2500843
TAGAGGG39300.067.207592
GGTAAGG14600.066.900311
GAATAGG14800.066.6338961
AAGGGAT36700.066.44274
AGAGGGA50250.066.359973
AGTAGGG37900.066.081232
ATAAGGG30150.065.8593752
GAGGGAT28400.065.765734
AGATAGG14300.065.664051
TAAGAGG16300.065.423151
AAGAGGG79900.065.169612
TATAGGG16550.064.9770052
Produced by FastQC (version 0.11.5)