FastQCFastQC Report
Mon 27 Feb 2023
SRR3128957.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128957.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260783
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT369461.634212571485189No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG31490.13928802543189683No Hit
CGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT24730.10938688056306156No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC23630.10452130965245227No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT151750.086.549961
GAATAGG12550.073.8683241
TAGGGCA10900.073.727354
AGGGATG40100.072.427445
GTAGGGC10300.070.265563
ATAGGGC10600.069.606993
AGGGTAC10950.069.528045
TAGAGGG41250.069.41822
AGTAGGG34650.068.2566152
TAAGGGA18250.067.982963
GGTAAGG13650.067.570811
GTAGGGA17000.067.1763463
AAGGGTA14100.066.32744
AAGAGGG83550.065.675652
TAGACGG2800.065.545421
ACGGGTA2100.064.898964
GAGGGAT28750.064.8950654
AGAGGGC35100.064.803323
AGAGGGA54050.064.429023
TAAGAGG15850.064.426741
Produced by FastQC (version 0.11.5)