FastQCFastQC Report
Mon 27 Feb 2023
SRR3128956.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128956.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2410201
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380221.5775447773857865No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG33370.13845318295030165No Hit
CGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT26070.1081652526075626No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT161150.086.5460361
GAATAGG13900.072.418871
AGGGATG38900.071.882565
TAGAGGG44050.069.453032
GGTAAGG13600.068.4824141
ATAGGGC12300.068.393233
AGGGTAC11150.068.280445
ATAGGGA19700.067.989813
GTAGGGC10900.067.2609943
TAGGGCA11400.067.196694
GAGGGAT29800.067.182864
TATAGGG19800.066.932972
AGTAGGG41400.066.6337052
AAGAGGG88500.065.9529952
CGTAGGG7200.065.9242252
AGAGGGA54950.065.769393
TAAGAGG16250.065.130131
ACGGGAT8150.064.58414
AGAGGGC34750.064.5102543
GTAGGGA15250.064.4082643
Produced by FastQC (version 0.11.5)