FastQCFastQC Report
Mon 27 Feb 2023
SRR3128954.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128954.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2214543
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241981.0926859401691456No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG25050.11311588892155176No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC23890.1078777878776795No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT103400.084.225891
ATAGGGC13200.076.191943
ACGGGTA1650.074.055724
GAATAGG12550.071.9808961
ATAGCGG5250.071.695251
GGTAAGG13350.071.191771
ATAACGG2450.071.055121
AGATAGG12050.070.672631
TAGGGAT11900.069.113084
ATAAGGG31350.068.685312
TAGAGGG41050.068.489492
ATAGAGG14800.068.349681
AAGGGGC74100.067.990114
AGGGATG29050.067.461985
GATAGGG32800.066.7956162
TAGGGCA10800.066.578934
TATAGGG19400.066.402542
AGAGGGC32550.066.2721253
AAGAGGG87600.066.228812
AAGGGTA12750.065.979954
Produced by FastQC (version 0.11.5)