FastQCFastQC Report
Mon 27 Feb 2023
SRR3128953.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128953.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332088
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250381.073630154608231No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG26620.11414663597600092No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC25330.10861511229421875No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT108000.084.899361
GAATAGG14100.073.070371
AGATAGG14100.071.7357561
TAGAGGG40700.069.858752
GGTAAGG17300.069.344241
ATAGGGC12400.068.980793
AGGGATG31300.068.016415
AAGGGGC84250.067.7215964
GGATAGG12650.067.6857451
AAGAGGG88300.067.593182
ATAGGGT8000.067.559483
CGTAGGG6400.067.556582
GATAGGG33400.067.3982852
AGAGGGC33400.067.260473
AGGGATA18800.066.744155
ATAGGGA17850.066.3499763
TAGGGTA5950.066.3499764
AATAGGG36150.066.301252
ATAGAGG15050.066.2698061
TAGGGCA9950.065.655364
Produced by FastQC (version 0.11.5)