FastQCFastQC Report
Mon 27 Feb 2023
SRR3128952.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128952.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2344882
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212680.9069965994024434No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG37000.15779045598030092No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC35970.15339791085436283No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG25920.11053861132457837No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86100.081.902951
CGTAGGG6600.075.7688752
ATAGGGC14900.074.406433
TAGGGCA12000.073.517254
AGGGATG36650.073.340465
ATAGGGA20550.072.774023
GAGGGAT27100.072.244994
TAAGGGA18900.071.38933
ACGGGAT7400.068.852694
AAGGGAT36000.068.799574
ATGTAGG5700.068.731421
TATAGGG18300.068.5742
TAGAGGG40950.067.971532
AGTAGGG37800.067.270682
AGATAGG13950.066.99511
AGTAAGG16750.066.7860261
AGGGTAC11400.066.777755
ATAGAGG14450.066.636851
CGAGGGA14450.066.602613
AAGAGGG83950.066.42412
Produced by FastQC (version 0.11.5)