FastQCFastQC Report
Mon 27 Feb 2023
SRR3128951.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128951.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2427729
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211170.8698252564433675No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC39630.1632389776618395No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG38980.1605615783310246No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG27420.11294506100145445No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86800.082.125061
AGGGATG40550.072.4372255
ATAGGGC15250.071.8056643
CGTAGGG7300.071.5072562
ATAGGGA19850.071.5021443
TAGGGTA5750.071.108924
TAGAGGG45600.070.334682
TAGGGCA10250.069.693514
AAGGGAT39400.069.6609344
ATAGCGG5100.069.194861
AGGGTAC11900.069.113635
GTACGGG6300.068.674782
AGGGCAT21300.067.9584965
AGATAGG15500.067.69491
AGTAGGG41900.067.678912
ACGGGAT7500.067.676074
GAGGGAT29200.066.955024
AGTAAGG15350.066.823751
TAGGGAT14400.066.579474
TAAGGGA20050.066.33533
Produced by FastQC (version 0.11.5)