FastQCFastQC Report
Mon 27 Feb 2023
SRR3128950.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128950.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2549948
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216300.8482525918175587No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC43280.16972895133547822No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG40380.1583561704003376No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG29760.1167082622861329No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86700.081.351451
TAGGGTA8200.073.358524
ATAGGGC16100.073.265973
AGGGATG42600.072.147725
ATGTAGG7100.071.572831
AGGGTAC12150.071.556635
CGTAGGG8500.071.322282
TAGAGGG46600.070.392112
TAGGGCA12100.070.298764
AAGGGAT38450.069.5457154
GAGGGAT31350.069.256234
GTAGGGT9850.069.18093
ATAGAGG18650.069.1280751
TAAGGGA20150.068.568833
ATAGGGA20800.067.329813
GCATAGG7050.066.741151
AAGGGGC81300.066.128694
AGAGGGC36400.065.845073
AGTAGGG43600.065.4269562
AAGAGGG90800.065.369022
Produced by FastQC (version 0.11.5)