FastQCFastQC Report
Mon 27 Feb 2023
SRR3128949.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128949.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2483494
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312621.2587910419755393No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG32510.13090428243434452No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC29850.12019356600015946No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT129200.085.256911
ATAGGGC11050.077.262813
TAGGGTC5750.071.368354
AGATAGG14000.069.780421
ATAGAGG15900.069.159221
AGGGATG38650.069.059545
GAATAGG14350.068.736211
ATAGGGA21000.068.282363
AATAGGG37650.067.777662
ATAGGGT10500.067.38393
TAGAGGG43000.067.352562
AAGAGGG94000.067.290722
TAAGAGG18400.067.200841
TAAGGGA19100.066.925273
TAGGGCA10400.066.671194
ATAGCGG5750.066.4826661
AGGGAAT21400.066.3159565
AGGGTAC13050.066.257065
GGTAAGG15400.066.194861
GATAGGG38400.065.839682
Produced by FastQC (version 0.11.5)