FastQCFastQC Report
Mon 27 Feb 2023
SRR3128947.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128947.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2639611
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316471.1989266600267994No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG36920.1398690943476141No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC34110.12922358635420142No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT127550.084.937251
ATAGGGC12800.078.5781253
TAGGGCA11550.073.653684
AGGGATG37600.071.7472845
GAATAGG15150.071.106221
GTACGGG5900.070.896962
ATAGAGG16950.069.938381
ATAGGGA20700.069.9323653
AGATAGG15850.069.153051
GGTAAGG14250.068.994761
AGTAGGG43900.068.089822
TAGGGAT13900.067.287774
AAGAGGG98600.066.2563552
TAGAGGG44850.066.22842
TAAGAGG16800.065.8026351
GTAGGGT9800.065.704083
GAGGGAT29200.065.349314
GATAGGG42500.065.245822
AAGGGAT43050.065.068534
CGTAGGG6750.064.754332
Produced by FastQC (version 0.11.5)