FastQCFastQC Report
Mon 27 Feb 2023
SRR3128946.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128946.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2777672
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274700.988957659507674No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG39710.14296144397178645No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC37340.13442911906085384No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA2600.083.419164
CGTTTTT117700.081.765561
GTAGGGC15150.071.892013
AGTAGGG46500.071.282632
CGTAGGG6850.069.520382
GGTAAGG18800.068.5048751
AAGGGAT41900.068.418084
TAGGGCA14300.068.252044
ATAGGGC17500.067.895943
TAGGGCG5950.067.3570864
TAAGGGA22150.067.265863
AGTAAGG18900.067.1441
AGGGATG39650.067.0819855
ATAGCGG5500.066.9034651
TAGAGGG50750.066.05642
GTAAGGG46000.066.009942
GTAGGGA20200.065.823213
AAGAGGG99100.065.610292
ATAGGGA25900.065.536633
GTAGGGT11300.065.509243
Produced by FastQC (version 0.11.5)