FastQCFastQC Report
Mon 27 Feb 2023
SRR3128945.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128945.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2870497
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271240.9449234749243772No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG42030.1464206372624671No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC40200.1400454346407608No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT111100.082.012341
ATAGGGC17450.074.3341453
TAGGGCA14200.073.474954
CGTAGGG7350.072.306292
ACGGGTA2550.071.878524
GGTAAGG17300.069.9157261
GTAGGGC13550.069.3689963
ATAGCGG5750.068.754221
AGTAGGG45100.068.200282
GTAGGGT9750.067.483583
ATAGGGA27500.066.480093
AGGGCGA16350.066.400125
AGGGAAT28400.066.359135
AGAGGGC44050.066.041883
AAGAGGG104550.066.036942
TAGAGGG50300.066.011822
TAGGGCG6800.065.6582644
GAGGGAT34400.064.894794
ATGCGGG14250.064.358322
GTAGGGA21150.064.2187963
Produced by FastQC (version 0.11.5)