FastQCFastQC Report
Mon 27 Feb 2023
SRR3128944.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128944.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2975149
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279000.9377681588384312No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG44030.14799258793425135No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC42720.14358944711676624No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT116500.083.158561
CGTAGGG9000.073.103172
TAGGGCA15600.072.298634
ACGGGTA2500.071.431054
GTAGGGA21950.069.3683243
TAGAGGG52600.069.241532
ATAGGGC18200.069.201293
GTAGGGC14600.068.561063
AGGGATG42950.068.166085
GAGGGAT36800.067.809434
AGTAGGG46550.067.438442
GAATAGG18050.067.254151
ATAAGGG38150.066.8892062
ATAGGGA26350.066.880783
AGATAGG18450.066.5611341
AGTAAGG18700.066.426121
TAAGGGA24550.066.233133
ATAGCGG6600.065.58741
ATGAGGG48150.065.4902952
ACGGGAT7500.065.165164
Produced by FastQC (version 0.11.5)