FastQCFastQC Report
Mon 27 Feb 2023
SRR3128943.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128943.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2155027
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163030.7565102432591332No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG32540.1509957879878071No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28120.13048560412468152No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG27800.12900070393549593No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC27420.12723738496083808No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62650.079.196221
ATAGGGC14650.075.941993
TAGGGCA10250.074.967214
AGGGATG36150.073.577195
AGTAAGG12350.072.177971
TAAGGGA19300.071.3235863
GAATAGG13200.070.745871
GGTAAGG12200.070.7458651
AAGAGGG76500.069.2647862
TAGAGGG40750.068.023252
AGTAGGG32700.067.757552
GACCGAT1250.067.670218
GAGGGAT28250.067.083424
CGTAGGG5700.066.9911962
ATAGGGA18750.066.878723
GTACGGG6350.066.815372
AAGGGGC68800.065.847994
TAGGGTA5550.065.404164
AAGGGAT34750.065.252614
AGAGGGC33050.065.18573
Produced by FastQC (version 0.11.5)