FastQCFastQC Report
Mon 27 Feb 2023
SRR3128942.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128942.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2205498
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162890.7385633539454581No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG34960.1585129526301996No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC29840.1352982410321841No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG28900.13103616507473595No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28530.1293585394319106No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65750.079.480131
TAGGGCA9650.074.0273364
ATAGGGC12700.072.531763
TAAGGGA18850.070.808083
AGGGATG33400.069.5114445
ATAAGGG30950.069.4301452
ATAGGGA17900.069.3149263
GAATAGG11750.069.2545851
AGTAGGG34400.068.207892
ACGGGTA2150.067.764014
CGTAGGG6600.067.681852
AGGGAAT20000.067.6765755
AAGAGGG81200.067.636222
GGTAAGG11950.067.308291
AGGGCAT18250.066.440475
AGAGGGA51850.066.077653
TAGAGGG40100.066.017112
ATAGAGG13950.065.750751
GGATAGG13700.065.577231
TATAGGG16600.065.432932
Produced by FastQC (version 0.11.5)