FastQCFastQC Report
Mon 27 Feb 2023
SRR3128941.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128941.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2325043
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167730.7214060127060016No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG36200.15569604519142227No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG31710.1363845743928177No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC31430.1351802955902321No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29160.1254170352978418No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66750.079.0734561
ACGGGTA2050.077.9476554
GGTAAGG12950.074.468611
GAATAGG14600.072.4967961
AGTAGGG35450.071.988242
AGGGCAT18800.071.740545
GTAGGGT8650.071.1759343
ATAGGGC15500.069.738753
TAAGGGA18000.069.713483
AGGGATG34650.069.439625
TAGGGTC6800.068.423344
AGATAGG14050.068.303511
CGTAGGG7100.068.179982
TAGGGCA11800.068.1070564
ATAAGGG28950.068.0210652
TATAGGG18200.067.9144742
AAGAGGG85200.067.6283652
TAAGAGG17800.067.392151
ATAGGGA19450.067.174313
ATAGAGG14750.066.337711
Produced by FastQC (version 0.11.5)