FastQCFastQC Report
Mon 27 Feb 2023
SRR3128940.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128940.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2436806
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460281.888865999180895No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG50800.20846961145039858No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT40670.16689880113558486No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT39160.16070216504719703No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC36590.1501555724994111No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCT35600.14609287731563367No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT174700.088.0603941
AGGGATG33250.073.777175
ATAGCGG4550.072.6011
ATAGGGC13750.071.697293
ATAGGGA20600.071.440843
TAGGGCA10250.069.488384
TAGGGTA7400.069.479064
ATAAGGG33150.069.153142
GAATAGG14450.068.254991
AAGGGAT34650.067.793064
GGTAAGG14250.067.5571441
AGGGTAT10550.067.2616655
TATAGGG20200.066.784212
AGGGAAT21200.066.7227255
GATAGGG36400.066.477572
ATAGAGG16000.066.3618551
AGATAGG14800.066.321991
TAGAGGG43150.065.916732
AATAGGG35900.065.826752
AAGGGTA13900.065.494024
Produced by FastQC (version 0.11.5)