FastQCFastQC Report
Mon 27 Feb 2023
SRR3128939.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128939.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2478305
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT453501.8298796960019046No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG51230.20671386290226587No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT42750.1724969283441707No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT42430.17120572326650674No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC38540.15550951154115414No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCT36070.1455430223479354No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT174800.087.465751
ATAGGGA21650.074.024223
ATAGGGC12950.073.309283
GGTAAGG14900.072.639241
AGGGATG33650.071.0902565
TAGGGTA7650.069.421564
TACGGGT1500.068.930083
GAGGGAT26400.068.360414
TAAGGGA20250.068.233813
GAATAGG15600.068.173181
ACGGGTA2050.066.484674
ATAGAGG15100.066.379251
AGATAGG15150.066.160181
TATAGGG19650.066.04682
CGTAGGG7200.065.9620362
AGAGGGA49900.065.928753
AAGGGAT37050.065.708094
TAGGGCA11350.065.4242254
AAGAGGG88800.065.079492
GATAGGG36650.064.792192
Produced by FastQC (version 0.11.5)