FastQCFastQC Report
Mon 27 Feb 2023
SRR3128937.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128937.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2246050
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151570.6748291444981189No Hit
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG25850.11509093742347676Illumina PCR Primer Index 8 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC23410.10422742147325305Illumina PCR Primer Index 8 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68850.080.559861
CGTAGGG6650.077.270982
ATAGGGC13350.074.50973
AGGGATG33600.072.587975
TAAGGGT9550.071.5774463
GAATAGG15850.071.118711
TATAGGG19600.070.7135852
ACGGGTA2350.070.212074
ATAGGGA20200.070.013453
GAGGGAT29600.069.2802054
ATAAGGG32750.069.0941852
AGGGTAC12200.068.17895
AATAGGG35200.067.9011152
TAGAGGG40250.067.580512
AAGAGGG85700.067.385562
TAGGGCA10850.067.346274
AAGGGGC61400.066.1068344
TAAGGGA19750.065.64133
AGTAAGG14250.065.5338441
GGTAAGG15700.065.189161
Produced by FastQC (version 0.11.5)