FastQCFastQC Report
Mon 27 Feb 2023
SRR3128936.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128936.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2304526
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147260.6390034219618264No Hit
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG26150.11347235830708788Illumina PCR Primer Index 8 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC24960.10830860662886857Illumina PCR Primer Index 8 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG23190.10062806841840795Illumina PCR Primer Index 8 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT63750.080.147011
CGTAGGG7650.079.295592
ATAGGGC11650.075.0331653
GAATAGG15450.073.38821
ACGGGTA2600.072.302444
TATAGGG16200.071.697282
AGGGATG32800.071.497855
ATAAGGG33350.069.372932
TAGAGGG40150.068.984342
AGGGCAT17200.067.762515
TAAGGGA20550.067.6933753
AGGGTAC11150.067.439055
AATAGGG34500.067.33312
AAGGGAC22650.067.019414
AGTAAGG14850.066.8488161
CGAAGGG26600.066.646982
GTAGGGA16850.066.380933
AAGAGGG88550.065.902822
TAGGGCA10000.065.325254
AGAGGGC35200.065.154363
Produced by FastQC (version 0.11.5)