FastQCFastQC Report
Mon 27 Feb 2023
SRR3128935.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128935.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2457253
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155010.6308263740038165No Hit
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG27910.1135821179178538Illumina PCR Primer Index 8 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC26730.10878000759384565Illumina PCR Primer Index 8 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG25150.10235006326169914Illumina PCR Primer Index 8 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70750.078.67191
ACGGGTA2050.073.357514
AGGGTAC12400.071.62755
CGTAGGG6750.070.317932
TAGGGTA6850.069.29144
ATAGCGG5850.068.3635251
GAGGGAT32000.068.2891164
ATAAGGG35150.068.185712
TAGAGGG41950.067.7753752
TAAGGGT10900.067.689553
AAGAGGG92350.067.527792
AGGGATG35800.067.208875
GAATAGG16650.066.972341
TGTAGGG19350.066.788272
TAAGGGA21700.066.485533
GAGGGTA14650.066.081224
ATAGAGG16300.065.812531
AGTAGGG41750.065.623682
ATAGGGA23900.065.477913
TAGCGGG15550.065.2787552
Produced by FastQC (version 0.11.5)