FastQCFastQC Report
Mon 27 Feb 2023
SRR3128934.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128934.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987859
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115081.1649435800048389No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55550.085.010931
ACGGGAT2050.073.6075444
ATAGGGA8900.071.526993
ACGGGTA1000.070.7322544
AAGGGTA6950.070.562634
AGGGTAC5600.069.649655
AGTAAGG6950.069.2373051
ATAGCGG1450.068.324441
TAGAGGG18350.067.327332
AGTAGGG16950.067.32432
TAGGGAT6200.066.1688844
AGGGCAT6750.065.441465
AAGAGGG39000.065.2913062
GAGGGTA5850.065.2913064
GTACGGG2100.065.1185762
TAGGGTA3050.064.9345254
GACCGAT800.064.614748
TAACGGG2850.064.5276642
TAAGGGA8200.064.40663
TAGGGCA5200.064.384484
Produced by FastQC (version 0.11.5)