FastQCFastQC Report
Mon 27 Feb 2023
SRR3128933.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128933.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991618
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111781.1272485977463096No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55750.084.49331
ATAGGGC5700.071.7347643
TATAGGG7450.071.333282
AGGGATG13250.071.296055
TAGGGAT6300.070.8709644
GTAGGGA6800.069.80683
AGTAGGG15950.069.291062
ATAGGGA9100.069.206793
CGTAGGG2800.068.864582
TAGAGGG18250.067.773972
TAATAGG3550.067.604391
AGGGTAC4500.066.842515
GAATAGG6950.065.678021
AAGGGAT14350.065.503334
AGGGTAT6200.065.1916665
GACCGAT651.0913936E-1165.0750358
TAGGGCA5000.064.8581164
AATAGGG16650.064.400712
ATAAGGG13200.064.1310962
ATGAGGG15800.063.9957922
Produced by FastQC (version 0.11.5)