FastQCFastQC Report
Mon 27 Feb 2023
SRR3128932.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128932.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034659
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115141.112830410792348No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT54650.084.170371
ATAGGGC5550.074.522133
ATAGGGA9100.072.307323
ATAGGGT4200.071.6186753
AGGGATG14900.071.284785
TATAGGG9050.068.552142
TAGGGAT6500.067.968884
AGTAGGG17400.067.79852
AATAGGG15900.066.804692
TAGGGCA5150.066.621024
GAGGGTA6300.066.3964844
ATAGAGG7250.066.171771
AGGGCAT6900.066.068935
TAGAGGG21350.066.041812
AGAAGGG37200.065.824932
GATAGGG15800.065.145232
AGTACGG1300.065.1237951
AAGAGGG40500.064.291032
AAGGGAT16000.064.037174
GAATAGG7000.063.8316571
Produced by FastQC (version 0.11.5)