FastQCFastQC Report
Mon 27 Feb 2023
SRR3128931.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128931.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences766652
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109891.4333752471786416No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC22580.29452737356714653No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG16790.21900418964536714No Hit
CGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT13250.172829393257958No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG12430.16213353646765416No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT51900.086.877971
TAGGGCA3700.073.924534
AGTACGG1350.073.367651
ACGGGTA900.073.358054
AGATAGG4250.072.134581
ATAGGGA5450.071.819753
AGGGCAT5400.071.359235
TAGGGTA2250.071.262114
ATAGGGC4400.070.738123
AGTAGGG10500.069.6152952
TAAGGGA6350.069.067153
AGGGATG13150.068.612865
AGAGGGT7900.068.0518653
AAGGGTA5400.067.244874
CGGGTAT700.067.132385
CGAAGGG7950.067.030672
TAAGAGG6200.066.9437561
AGGGAAT7250.066.761995
ATAAGGG10400.065.7501752
GTAGGGC3450.065.6121753
Produced by FastQC (version 0.11.5)