FastQCFastQC Report
Mon 27 Feb 2023
SRR3128930.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128930.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780700
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107311.374535673113872No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC23370.2993467401050339No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG17070.2186499295504035No Hit
CGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT13530.17330600742923016No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG12430.15921608812604074No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA650.086.7649154
CGTTTTT51700.086.544471
TAGGGCA3950.074.95834
AGGGTAT3300.074.056925
AGTAGGG11150.072.100222
GTACGGG2100.071.638422
TAAGAGG6000.071.3574751
AGGGATG12500.070.684485
ATAAGGG10000.070.519072
ACGGGTG1600.070.496494
TAGGGTA2200.070.496494
AAGGGTA5750.070.292164
AGTACGG1950.069.970141
AGAGGGC10150.069.454673
ATAGGGC4700.068.996563
AGAGGGT7450.068.761683
ATAGGGA6200.067.4643863
TAGAGGG12550.067.42862
AAGGGCA10500.067.139524
AAGAGGG28300.067.1135562
Produced by FastQC (version 0.11.5)