FastQCFastQC Report
Mon 27 Feb 2023
SRR3128929.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128929.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences809199
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109251.350100531513262No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC24890.3075881210925866No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG17690.21861124395853182No Hit
CGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT14210.17560575334373868No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG13990.17288701543130922No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT52100.086.790581
TAGGGCA3450.074.928534
AAGGGTA5500.074.3464364
GTAGGGC3600.073.112073
TAGACGG1000.070.579441
ATAGGGC4550.070.2426763
GGTAAGG4900.070.0993041
ATGCGGG4500.068.934242
CGAAGGG7850.068.854412
AGGGATG11800.068.111065
ATAAGGG10050.067.811842
TAAGGGA6100.067.804173
AGGGTAA3400.067.736195
AATAGGG10650.067.522012
TAAGAGG5650.067.456451
TAGCGGG4600.067.435672
TAGAGGG13350.067.244332
AGTTAGG2950.066.990651
AAGGGCG4000.066.975884
GTAGGGT3100.066.7105563
Produced by FastQC (version 0.11.5)