FastQCFastQC Report
Mon 27 Feb 2023
SRR3128928.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128928.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2286007
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168890.7387991375354495No Hit
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG26600.11636009863486857RNA PCR Primer, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC24610.10765496343624495RNA PCR Primer, Index 16 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71750.080.583491
TAGGGCA12050.073.152964
ATAGCGG5400.072.487281
ATAGGGC13000.071.070613
CGTAGGG6000.069.9223562
AGAGGGC32550.068.499513
ATAGGGA19750.068.2615663
TAGAGGG43450.067.91442
AGATAGG15050.067.685361
AGTAGGG36400.067.599962
AAGGGTA15750.067.340964
AGGGATG30350.067.199645
GGTAAGG14250.066.190031
GAATAGG13050.066.132971
ATAAGGG31650.066.128182
GAGGGAT30700.065.717764
ATGAGGG38850.064.914252
AGGGTAC10850.064.534575
AGGGCAT18300.064.4552845
GATAGGG35300.064.364982
Produced by FastQC (version 0.11.5)