FastQCFastQC Report
Mon 27 Feb 2023
SRR3128927.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128927.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2346848
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165670.7059255648427167No Hit
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG27630.1177323797706541RNA PCR Primer, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC26970.1149200970834072RNA PCR Primer, Index 16 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64700.081.372371
ATAGGGC13450.070.2318653
AGTAGGG38850.069.099052
AGGGATG33050.068.538735
TAGGGCA12400.067.461944
GAGGGAT31450.067.393244
GAATAGG14600.067.0288241
ATAGGGA20400.066.807973
ATAGAGG17650.066.6417541
TAGTAGG10450.066.1838761
GGTAAGG13350.065.903951
TAGAGGG42850.065.830542
GATAGGG40200.064.790412
AGGGTAC10600.064.286925
AGATAGG16250.064.276281
ATAAGGG33850.064.1667942
AAGGGGC70000.063.9144944
AGAAGGG87400.063.85082
ATGCGGG12750.063.7915232
AGAGGGC32350.063.7750363
Produced by FastQC (version 0.11.5)