FastQCFastQC Report
Mon 27 Feb 2023
SRR3128926.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128926.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2455949
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173240.705389240574621No Hit
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC30160.12280385301160571RNA PCR Primer, Index 16 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG28190.11478251380627204RNA PCR Primer, Index 16 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69950.078.960411
TAGGGCA12350.071.922344
ATAGGGC12900.070.313153
AGGGATG30300.068.553715
AGTAGGG42700.068.127572
GAGGGAT28900.067.81214
ATAGGGA19750.067.10443
GGTAAGG14700.066.5694661
CGTAGGG5800.066.44252
GAATAGG13750.065.694281
TAGAGGG45450.065.039452
ATAAGGG35200.064.6193852
AGAAGGG95800.064.116562
AAGGGGC75850.064.066944
AGGGTAC10950.063.947485
GTAGGGC11650.063.738253
TAGGGAT12300.063.4266744
AGATAGG16050.063.315171
AATAGGG36300.063.3085482
TATAGGG19450.063.305552
Produced by FastQC (version 0.11.5)