FastQCFastQC Report
Mon 27 Feb 2023
SRR3128925.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128925.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2624487
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202770.7726081325607633No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC29650.11297445938958736No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG29160.11110742785161443No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT73550.079.618831
AGGGTAC11600.070.489925
TAGGGTA8000.070.156464
AGGGATG34750.069.374265
AATAGGG41000.069.2505652
CGTAGGG6000.069.173882
TAGGGCA14600.069.130814
ATAGGGA21000.068.724713
AGTAGGG44800.068.324652
ATAGGGC14900.067.738923
GAATAGG16400.067.6161961
TAGAGGG47850.067.320842
ACGGGAT6250.065.6523064
GACCGAT2150.065.570788
AGGGTAG10550.065.478795
GTAGGGC12900.064.34783
ACGGGTA3750.064.143064
AAGAGGG97100.064.115852
ATGTAGG7400.063.7668531
TACGAGG6300.063.6656341
Produced by FastQC (version 0.11.5)