FastQCFastQC Report
Mon 27 Feb 2023
SRR3128924.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128924.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2667237
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198780.7452656063184486No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC30870.11573774658944819No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG30190.1131882918540797No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71700.080.285221
ATAGGGC14100.074.329673
TAGGGCA14150.071.742054
ATAGGGA20050.071.492743
AGGGTAC13100.070.317155
AGGGATG34550.069.37435
CGTAGGG6850.069.342652
TAGAGGG51300.069.123172
GAATAGG16350.068.803161
AAGGGTA15900.068.575244
AGTAGGG45600.067.75952
GTAGGGC10700.067.6415253
ATGAGGG43950.066.986172
ATAGAGG20800.066.529191
TAAGGGA23800.065.9547353
AATAGGG41700.065.638182
ACGGGTA3850.064.698114
ACGGGAT6850.064.493174
GAGGGAT28050.064.339084
AAGAGGG94000.064.140132
Produced by FastQC (version 0.11.5)