FastQCFastQC Report
Mon 27 Feb 2023
SRR3128923.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128923.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2792662
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204120.7309155207468716No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC34020.12181925345781193No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG32800.11745066177002443No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG28450.10187412583406083No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74600.080.461341
AGGGTAC13100.073.898515
ATAGGGA22750.071.474323
TAGGGCA13850.070.577934
AGGGATG33700.070.002515
TAGAGGG52550.069.934162
ATAGGGC13000.068.685583
AGTAGGG45200.068.62162
AATAGGG42700.066.585982
ACGGGTA2900.066.441774
AAGGGTA16350.066.109734
ATAGAGG20550.065.6971
CGTAGGG7800.065.673062
GAATAGG17800.065.540111
AGTACGG3450.065.448181
GATAGGG39850.065.21572
ATAGGGT10700.065.0029753
ATGAGGG44450.064.916022
TATAGGG19650.064.812992
ATAACGG2250.064.811881
Produced by FastQC (version 0.11.5)