FastQCFastQC Report
Mon 27 Feb 2023
SRR3128922.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128922.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2395864
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170930.7134378245175853No Hit
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG37610.15697886023580637TruSeq Adapter, Index 14 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC37560.15677016725490261Illumina PCR Primer Index 3 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG32460.13548348320271936TruSeq Adapter, Index 14 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA3450.090.230034
CGTTTTT76300.080.083751
CGTAGGG6500.079.093242
TAGGGCA14350.077.244
AGGGTAC17850.075.029975
ATAGGGC14050.073.182363
GTAGGGC11900.072.532123
TAGGGCG5200.071.655584
AGGGTAT15650.071.464575
GAGGGTA18950.071.4330144
AAGGGTA19350.071.17514
GGTAAGG14100.070.944031
AGGGCAT22750.070.437045
ATAGGGA21750.070.260623
AGGGATG31550.070.0046165
GTAGGGT9200.069.72323
ATAGCGG4000.069.59711
TAAGGGA20400.069.130123
AGTACGG1850.068.863831
AGTAAGG15150.068.830161
Produced by FastQC (version 0.11.5)