FastQCFastQC Report
Mon 27 Feb 2023
SRR3128921.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128921.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2478003
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169830.6853502598665134No Hit
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC41990.169450965152181Illumina PCR Primer Index 3 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG39690.16016929761586246TruSeq Adapter, Index 14 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG34730.14015317979841024TruSeq Adapter, Index 14 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74600.080.362871
AGGGTAC16100.080.276425
ATAGGGC12900.074.687063
TAGGGCA13350.073.929744
ACGGGTA3500.072.51154
CGTAGGG5350.072.073882
AGGGCAT24450.071.5064545
AAGGGTA22550.071.4872744
TAGGGCG5950.071.089714
CGAAGGG25100.071.004082
TAAGGGA20200.070.497293
ATAGGGA21300.069.504373
TAGGGTA8900.069.177124
AGTAAGG14900.068.848591
GATAGGG36550.068.831032
AGGGTAT15500.068.5264055
GGTAAGG14200.068.265881
AGATAGG14300.067.78851
AGAGGGC39300.067.6869663
GAATAGG15800.067.60731
Produced by FastQC (version 0.11.5)