FastQCFastQC Report
Mon 27 Feb 2023
SRR3128920.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128920.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2612007
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174750.669025772136139No Hit
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC44380.1699076610437874Illumina PCR Primer Index 3 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG42380.1622507137232021TruSeq Adapter, Index 14 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG34390.1316612091774639TruSeq Adapter, Index 14 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75300.079.872531
ACGGGTA3000.078.328464
ATAGGGC16150.074.4969253
AGGGTAC18350.074.270475
TAGGGCA14850.073.423054
AGGGTAT14800.072.398135
CGTAGGG6700.072.2492452
TAGGGTA10950.072.105114
AAGGGTA20000.071.905544
GTAGGGC11500.071.5172963
TAGGGCG6450.071.406424
GTAGGGT10900.071.142373
GGTAAGG16050.070.9580841
GACCGAT2800.070.492928
TAAGGGA21650.069.898673
AGATAGG14400.069.284311
GAATAGG16950.068.856271
TAGGGTC8100.068.464884
ATAGGGA21150.068.440193
AGGGATG33050.067.400195
Produced by FastQC (version 0.11.5)