FastQCFastQC Report
Mon 27 Feb 2023
SRR3128919.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128919.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2172312
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275231.2669911136153555No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG28240.12999974221014293No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC24390.11227668953630972No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT110750.084.489931
CGTAGGG6000.075.4373862
AGTACGG2600.070.7454151
ATAGAGG15900.070.59711
TATAGGG17400.069.638672
TAGAGGG40900.069.512132
AGTAGGG34400.068.940772
ATAGGGC12000.068.758033
ATAGGGA20100.068.2595753
AGGGATG30600.067.5718845
TAGGGCA9700.067.563114
GTAAAGG16050.067.2926251
TATGCGG2250.067.077131
AAGGGGC70300.066.59794
AAGAGGG80750.065.920122
GTAGGGA15650.065.676313
AGGGTAT10600.065.613115
AGATAGG12950.065.5555951
GAATAGG13250.065.4951251
AATAGGG34750.064.854442
Produced by FastQC (version 0.11.5)