FastQCFastQC Report
Mon 27 Feb 2023
SRR3128918.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128918.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2198704
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266671.212850843042083No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG29680.13498861147294042No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC26270.11947947518174344No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT108550.084.6513441
GTAAAGG17050.070.368341
ATAGGGC13400.069.793613
CGTAGGG6000.069.749842
GAATAGG14750.069.5386051
TAGAGGG37750.069.505912
TATAGGG14700.069.094092
AGTAGGG36850.068.9065862
GTACGGG5800.068.101362
ATAGAGG15400.067.825611
AGGGATG30050.067.719055
GGTAAGG12850.067.371491
TAGGGCA10850.067.1381454
AATAGGG34050.067.115622
ATAGGGA17150.066.316053
GTAGGGC8450.066.18473
GTAGGGA18250.065.6666263
AAGAGGG81900.065.452382
TAGTAGG10600.064.804991
GAGGGAT24500.064.644444
Produced by FastQC (version 0.11.5)