FastQCFastQC Report
Mon 27 Feb 2023
SRR3128917.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128917.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2299991
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276481.2020916603586709No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG31230.13578314002098268No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC26430.11491349313975577No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT113100.083.475611
AGTAAGG16150.069.905851
ACGGGAT6150.069.538534
ATAGCGG4200.069.44121
AGGGATG30600.069.263575
ATAGGGA18100.068.546393
CGTAGGG5800.068.0629042
AATAGGG37350.067.819932
GAATAGG16250.067.449291
AGTAGGG40150.067.187042
TAGAGGG45050.067.181592
TAAGAGG14150.066.821231
AGAGGGC36650.066.6789253
ATAGGGC13050.066.26233
AAGGGAT32300.066.056044
AAGAGGG82600.065.998972
TAGGGAT12700.065.868154
ATAGAGG16000.065.857141
GTAGGGC8950.065.636613
TATAGGG17400.065.632092
Produced by FastQC (version 0.11.5)