FastQCFastQC Report
Mon 27 Feb 2023
SRR3128916.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128916.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2412634
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199830.8282648756504302No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC25290.1048231932402511No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG24450.10134152134140528No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA1900.081.908994
CGTTTTT80300.080.78151
AGGGATG39450.075.402565
TAGACGG3750.072.966171
ATAGCGG6600.072.194191
AAGGGTA19800.070.5013964
AGGGTAC12300.070.297865
TATAGGG17450.069.726122
ATAGGGC13600.069.005773
GAATAGG16900.068.950121
TAGGGCA11150.068.942024
AGGGCAT16800.068.810585
AGTAGGG42450.068.323272
GGTAAGG13950.067.97461
TAGTAGG11750.067.8537141
AGGGTAT12650.067.609895
ATAGGGA21250.066.1345753
AGACGGG16850.066.05162
GAGGGAT30950.065.673154
ATGAGGG39750.065.608362
Produced by FastQC (version 0.11.5)