FastQCFastQC Report
Mon 27 Feb 2023
SRR3128915.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128915.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2451027
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190610.7776740117509925No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC26830.10946431842652081No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG26530.1082403417016622No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT83150.079.719361
ACGGGTA2450.078.6483464
GGTAAGG13050.073.9023741
GAATAGG17600.072.438871
TAGACGG2700.071.4389651
AGGGATG37050.071.415435
ATAGGGC12900.071.042263
ATAGCGG5900.070.169071
ACGGGAT7150.069.674154
CGTAGGG6950.068.327342
AGGGCAT18300.068.056045
AGGGTAT12750.067.823395
AAGGGTA16450.067.710234
AATAGGG40750.067.381882
TAGGGTC8250.067.2202154
ATGAGGG38100.066.515332
TAGGGCA12750.066.348974
AGTAGGG40050.066.211672
AAGAGGG87500.065.985462
AAGGGAC24300.065.950784
Produced by FastQC (version 0.11.5)