FastQCFastQC Report
Mon 27 Feb 2023
SRR3128913.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128913.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2336555
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312571.337738679380541No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG33380.14285989416041994No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC26950.11534074738236422TruSeq Adapter, Index 27 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT119450.084.458041
ATAGCGG5450.073.55951
ATAGGGC13750.072.360713
AGGGATG36650.071.162735
TAGGGCA10850.070.840394
TATAGGG18000.068.635992
ATAGGGA20450.068.022383
GTAGGGC9250.067.800523
AGTAAGG14200.067.093341
AAGGGGC77750.066.956354
AGTAGGG35100.066.9029542
TAGAGGG43150.066.879612
TAGACGG3250.066.756081
GTAGGGT9000.066.540233
GGATAGG15550.066.121461
TAAGGGA19300.065.967423
AGGGCAT20000.065.790275
GAATAGG14950.065.304861
GATAGGG35750.065.290842
AGACGGG16050.065.223042
Produced by FastQC (version 0.11.5)